Journal: iScience
Article Title: USP18 promotes clear cell renal cell carcinoma progression by regulating the ubiquitination and stability of YBX3
doi: 10.1016/j.isci.2026.115808
Figure Lengend Snippet: USP18 deficiency inhibits the growth of ccRCC organoids (A and B) Bright-field (A, scale bars, 500 μm) and H&E (B, scale bars, 100 μm) images showing the morphology and structure of ccRCC organoids from three patient samples. (C and D) IF data show the expression of CK7 (C) and Vimentin (D) in ccRCC organoids (scale bars, 100 μm). (E) Bright-field images showing the morphology of control and USP18-deficient ccRCC organoids. “Before” indicates the bright-field image of the organoids after transfection with shNC/shUSP18 at 72 h, and “after” indicates the bright-field image of the organoids after transfection with shNC/shUSP18 at 96 h. The images taken before and after are of the same organoids at different time points in the same field of view. (F) Change in diameter (%) of control and USP18-deficient ccRCC organoids. (G) ATP assay indicating the viability of control and USP18-deficient ccRCC organoids. (H and I) Western blot data indicate the expression of USP18, YBX3, P-AKT, and P-PI3K in control and USP18-deficient ccRCC organoids. Unpaired two-tailed Student’s t test was employed for p -value calculation. Data are represented as mean ± SD.
Article Snippet: Inverted bright-field microscope , Motic , N/A.
Techniques: Expressing, Control, Transfection, ATP Assay, Western Blot, Two Tailed Test